New pubished article: Penickova et al. 2024

Congratulations to Dr Penickova and colleagues on the publication of their study on the bromocresol green colorimetric method for albumin determination in the Scandinavian Journal of Clinical and Laboratory Investigation. We are delighted to have contributed to the success of this article!

𝐀𝐥𝐛𝐮𝐦𝐢𝐧 is a protein produced by the liver, 60% of which is found in the tissues and 40% in the blood. It can also come from exogenous sources via the diet. Plasma albumin levels are routinely analyzed, particularly in cases of 𝐬𝐮𝐬𝐩𝐞𝐜𝐭𝐞𝐝 𝐤𝐢𝐝𝐧𝐞𝐲 𝐨𝐫 𝐥𝐢𝐯𝐞𝐫 𝐝𝐢𝐬𝐞𝐚𝐬𝐞. Several methods are available, including 𝐢𝐦𝐦𝐮𝐧𝐨𝐭𝐮𝐫𝐛𝐢𝐝𝐢𝐦𝐞𝐭𝐫𝐲 𝐚𝐧𝐝 𝐜𝐚𝐩𝐢𝐥𝐥𝐚𝐫𝐲 𝐞𝐥𝐞𝐜𝐭𝐫𝐨𝐩𝐡𝐨𝐫𝐞𝐬𝐢𝐬. The authors assessed the 𝐜𝐥𝐢𝐧𝐢𝐜𝐚𝐥 𝐫𝐞𝐥𝐢𝐚𝐛𝐢𝐥𝐢𝐭𝐲 of albumin determination using 𝐛𝐫𝐨𝐦𝐨𝐜𝐫𝐞𝐬𝐨𝐥 𝐠𝐫𝐞𝐞𝐧 compared with the other two methods.

Plasma samples from 320 patients were studied, 227 of whom 𝐡𝐚𝐝 𝐡𝐲𝐩𝐨𝐚𝐥𝐛𝐮𝐦𝐢𝐧𝐞𝐦𝐢𝐚. The results provided by the bromocresol green method 𝐰𝐞𝐫𝐞 𝐡𝐢𝐠𝐡𝐞𝐫 than those indicated by immunoturbidimetry and capillary electrophoresis, particularly in patients with 𝐞𝐥𝐞𝐯𝐚𝐭𝐞𝐝 𝐯𝐚𝐥𝐮𝐞𝐬 of C-reactive protein, alpha 1 globulin and alpha 2 globulin. Patients classified as having 𝐥𝐨𝐰 𝐡𝐲𝐩𝐨𝐚𝐥𝐛𝐮𝐦𝐢𝐧𝐞𝐦𝐢𝐚 with the method tested were considered to have 𝐬𝐞𝐯𝐞𝐫𝐞 𝐡𝐲𝐩𝐨𝐚𝐥𝐛𝐮𝐦𝐢𝐧𝐞𝐦𝐢𝐚 with the two reference methods.

As a result, the bromocresol green method 𝐚𝐩𝐩𝐞𝐚𝐫𝐬 𝐭𝐨 𝐨𝐯𝐞𝐫𝐞𝐬𝐭𝐢𝐦𝐚𝐭𝐞 plasma albumin concentrations in patients with inflammation and hypoalbuminemia. These data are important because, depending on the assay method used, the results of the analyses could lead to 𝐢𝐧𝐚𝐩𝐩𝐫𝐨𝐩𝐫𝐢𝐚𝐭𝐞 𝐦𝐞𝐝𝐢𝐜𝐚𝐥 𝐝𝐞𝐜𝐢𝐬𝐢𝐨𝐧𝐬.